論文名 Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae
記事種別等 Article
著者名 著者名(別言語) 上田, 裕貴 / 田原, 悠平 / 宮田, 真人 / 荻田, 亮 / 山口, 良弘 / 田中, 俊雄 / 藤田, 憲一
本文(PDF) ダウンロード回数:
回
この文献へのリンクには次のURLを使用してください 抄録・内容(英) Nagilactone E, an antifungal agent derived from the root bark of Podocarpus nagi, inhibits 1,3-β glucan synthesis; however, its inhibitory activity is weak. Anethole, the principal component of anise oil, enhances the antifungal activity of nagilactone E. We aimed to determine the combinatorial effect and underlying mechanisms of action of nagilactone E and anethole against the budding yeast Saccharomyces cerevisiae. Analyses using gene-deficient strains showed that the multidrug efflux pump PDR5 is associated with nagilactone E resistance; its transcription was gradually restricted in cells treated with the drug combination for a prolonged duration but not in nagilactone-E-treated cells. Green-fluorescent-protein-tagged Pdr5p was intensively expressed and localized on the plasma membrane of nagilactone-E-treated cells but not in drug-combination-treated cells. Quick-freeze deep-etch electron microscopy revealed the smoothening of intertwined fiber structures on the cell surface of drug-combination-treated cells and spheroplasts, indicating a decline in cell wall components and loss of cell wall strength. Anethole enhanced the antifungal activity of nagilactone E by enabling its retention within cells, thereby accelerating cell wall damage. The combination of nagilactone E and anethole can be employed in clinical settings as an antifungal, as well as a food preservative to restrict food spoilage.
備考 This research was partly funded by the Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (C) 16K08299 and 21K07029.
言語 eng
ページ開始 537
キーワード(日) キーワード(英) 著者版フラグ publisher
著者所属(英) Osaka City University / Osaka City University / Osaka City University / Osaka City University / Osaka City University / Osaka City University / Osaka City University
2人目の著者情報 : その他 3人目の著者情報 : KAKEN 3人目の著者情報 : その他 4人目の著者情報 : KAKEN 4人目の著者情報 : その他 5人目の著者情報 : KAKEN 5人目の著者情報 : その他 6人目の著者情報 : KAKEN 6人目の著者情報 : その他 7人目の著者情報 : KAKEN 7人目の著者情報 : その他 収録物名 巻 号 刊行年月 2021-05-06
出版社 MDPI
ISSN 2079-6382
資料種別 資料種別(英語) Journal Article
DOI https://doi.org/10.3390/antibiotics10050537
CCライセンス(BY) この作品はクリエイティブ・コモンズ 表示 4.0 国際 ライセンスの下に提供されています。
権利 © 2021 authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/